3D cell culture : methods and protocols

Other Authors: Haycock, John W.
Language:English
Published: New York: Humana Press/Springer, c2011..
Series:Springer protocols.
Methods in molecular biology (Clifton, N.J.)
Subjects:
Table of Contents:
  • 3D cell culture : a review of current approaches and techniques / John W. Haycock
  • Scaffolds for tissue engineering and 3D cell culture / Eleonora Carletti, Antonella Motta, and Claudio Migliaresi
  • Tracking nanoparticles in three-dimensional tissue-engineered models using confocal laser scanning microscopy / Vanessa Hearnden, Sheila MacNeil, and Giuseppe Battaglia
  • Using immuno-scanning electron microscopy for the observation of focal adhesion-substratum interactions at the nano- and microscale in S-phase cells / Manus J. Biggs, R. Geoff Richards, and Matthew J. Dalby
  • 3D sample preparation for orthopaedic tissue engineering bioreactors / Sarah H. Cartmell ... [et al.]
  • Quantification of mRNA using real-time PCR and western blot analysis of MAPK events in chondrocyte/agarose constructs / David A. Lee ... [et al.]
  • Genetic modification of chondrocytes using viral vectors / Teresa Coughlan ... [et al.]
  • Stem cell and neuron co-cultures for the study of nerve regeneration / Paul J. Kingham, Cristina Mantovani, and Giorgio Terenghi
  • Production of tissue-engineered skin and oral mucosa for clinical and experimental use / Sheila MacNeil, Joanna Shepherd, and Louise Smith
  • Three-dimensional alignment of Schwann cells using hydrolysable microfiber scaffolds : strategies for peripheral nerve repair / Celia Murray-Dunning ... [et al.]
  • Encapsulation of human articular chondrocytes into 3D hydrogel : phenotype and genotype characterization / Rui C. Pereira ... [et al.]
  • Micro-structured materials and mechanical cues in 3D collagen gels / James B. Phillips and Robert Brown
  • Organotypic and 3D reconstructed cultures of the human bladder and urinary tract / Claire L. Varley and Jennifer Southgate
  • Ex vivo organ culture of human hair follicles : a model epithelial-neuroectodermal-mesenchymal interaction system / Desmond J. Tobin
  • Human endothelial and osteoblast co-cultures on 3D biomaterials / Ronald E. Unger ... [et al.]
  • Assessment of nanomaterials cytotoxicity and internalization / Noha M. Zaki and Nicola Tirelli
  • Practical aspects of OCT imaging in tissue engineering / Stephen J. Matcher
  • Osteogenic differentiation of embryonic stem cells in 2D and 3D culture / Lee Buttery ... [et al.]
  • 3D structuring of biocompatible and biodegradable polymers via stereolithography / Andrew A. Gill and Frederik Claeyssens
  • Alvatex? : polystyrene scaffold technology for routine three dimensional cell culture / Eleanor Knight ... [et al.]